The Influence of Density Gradient Technique and Autologous Platelet Rich Plasma Mixed with Glycyrrhiza glabra Medium on DNA Fragmentation Index of Teratozoospermic Men
DOI:
https://doi.org/10.28969/IJEIR.v14.i2.r1.24Keywords:
DNA fragmentation index, PRP, Density gradient technique, Teratozoospermia, Glycyrrhiza glabraAbstract
Abstract
Background: Male infertility is a global health issue affecting over 20 million males. A spermatogenesis-related cell differentiation abnormality causes teratozoospermia (TZS). Extensive research has linked TZS to poor fertilization outcomes at assisted reproductive technology (ART) centers as well as to spontaneous pregnancies.
Objective: This study used Glycyrrhiza glabra (Gg) and Platelet Rich Plasma (PRP) media to establish a culture medium that aimed to improve certain sperm functions of teratozoospermic men in vitro.
Methods: A total of fifty semen samples were examined. The World Health Organization 2021 and 1999 standards were used to evaluate certain sperm parameters and the DNA fragmentation index. Platelet Rich Plasma and Gg extract were utilized as a medium for in vitro preparation and activation of semen samples complaining of teratozoospermia factor.
Results: Following activation by the utilization of the density gradient approach and the combination of Gg and PRP medium, there was a statistically a significant (p <0.001) improvement in certain sperm function metrics. These improvements included a higher percentage of morphologically normal sperm, a rise in active motility, and a drop in the DNA fragmentation index.
Conclusion: The density gradient technique, regardless of the medium used in this investigation, resulted in a significant enhancement of morphologically normal sperms with other certain sperm parameters and a decrease in the DNA fragmentation index.
Keywords: Teratozoospermia, DNA fragmentation index, Density gradient technique, Platelet Rich Plasma, Glycyrrhiza glabra
Downloads
References
Salonia A, Bettocchi C, Carvalho J, Corona G, Jones TH, Kadioglu A, Martinez-Salamanca JI, Minhas S, Serefoglu EC, Verze P. EAU guidelines on sexual and reproductive health. European association of urology guidelines. 2020.
https://doi.org/10.1038/s41443-020-0335-
Candela L, Boeri L, Capogrosso P, Cazzaniga W, Pozzi E, Belladelli F, Baudo A, Ravizzoli A, Ventimiglia E, Viganò P, Alfano M. Correlation among isolated teratozoospermia, sperm DNA fragmentation and markers of systemic inflammation in primary infertile men. Plos one. 2021 Jun 7;16(6):e0251608.
https://doi.org/10.1371/journal.pone.0251608
Mehrparvar B, Chashmniam S, Nobakht F, Amini M, Javidi A, Minai-Tehrani A, Arjmand B, Gilany K. Metabolic profiling of seminal plasma from teratozoospermia patients. Journal of pharmaceutical and biomedical analysis. 2020 Jan 30;178:112903.
https://doi.org/10.1016/j.jpba.2019.112903
Ammar, O., Mehdi, M. and Muratori, M., 2020. Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress. Andrology, 8(5), pp.1095-1106.
https://doi.org/10.1111/andr.12778
Braham A, Ghedir H, Zidi I, Sallem A, Hajlaoui A, Ajina M, Saad A, Ibala‐Romdhane S. Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome. Andrologia. 2019 Jun;51(5):e13252.
https://doi.org/10.1111/and.13252
Del Giudice F, Belladelli F, Chen T, Glover F, Mulloy EA, Kasman AM, Sciarra A, Salciccia S, Canale V, Maggi M, Ferro M. The association of impaired semen quality and pregnancy rates in assisted reproduction technology cycles: Systematic review and meta‐analysis. Andrologia. 2022 Jul;54(6):e14409.
https://doi.org/10.1111/and.14409
Dovere L, Maggiulli R, Stoppa M, Sanges F, Romano S, Albricci L, Rienzi L. Semen collection, analysis and preparation: A mini review. Current Trends in Clinical Embryology. 2016;3(2):41-6.
Nayar KD, Kant G, Gupta S. Semen Preparation Techniques. InAtlas of Assisted Reproductive Technologies 2023 Jun 27 (pp. 227-251). Singapore: Springer Nature Singapore.
https://doi.org/10.1007/978-981-99-0020-6_15
Aldhahrani A, Soliman MM, Althobaiti F, Alkhedaide A, Nassan MA, Mohamed WA, Youssef GB, Said AM. The modulatory impacts of Glycyrrhiza glabra extract against methotrexate-induced testicular dysfunction and oxidative stress. Toxicology Research. 2021 Aug;10(4):677-86.
https://doi.org/10.1093/toxres/tfab049
Ghorbanlou M, Rostamkhani S, Shokri S, Mahmazi S, Fallah R, Moradi F, Nejatbakhsh R. Possible ameliorating effects of (Licorice) on the sperm parameters in rats under high fat diet. Endocrine regulations. 2020;54(1):22-30.
https://doi.org/10.2478/enr-2020-0004
Al-Dujaily SS, NA H, SA B, SL S. Malondialdehyde measurements in semen after in vitro sperm activation by Pentoxifylline and Glycyrrhiza glabra extract. Inter J Res Pharm. Chemis. 2013;3(4):828-33.
Berrigan WA, Bailowitz Z, Park A, Reddy A, Liu R, Lansdown D. A Higher Platelet Dose May Yield Better Clinical Outcomes for PRP in the Treatment of Knee Osteoarthritis: A Systematic Review. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2024 Mar 19.
https://doi.org/10.1016/j.arthro.2024.03.018
Éliás M, Kónya M, Kekk Z, Turan C, das Virgens IP, Tóth R, Keszthelyi M, Hegyi P, Várbíró S, Sipos M. Platelet-rich plasma (PRP) treatment of the ovaries significantly improves fertility parameters and reproductive outcomes in diminished ovarian reserve patients: a systematic review and meta-analysis. Journal of Ovarian Research. 2024 May 17;17(1):104.
https://doi.org/10.1186/s13048-024-01423-2
Collins T, Alexander D, Barkatali B. Platelet-rich plasma: a narrative review. EFORT open reviews. 2021 Apr 1;6(4):225-35.
https://doi.org/10.1302/2058-5241.6.200017
World Health Organization. WHO laboratory manual for the examination and processing of human semen. World Health Organization; 2021.
World Health Organisation. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press; 1999 May 13.
Croisé B, Paré A, Joly A, Louisy A, Laure B, Goga D. Optimized centrifugation preparation of the platelet rich plasma: Literature review. Journal of Stomatology, Oral and Maxillofacial Surgery. 2020 Apr 1;121(2):150-4.
https://doi.org/10.1016/j.jormas.2019.07.001
Mohammed EH, Abu-El-Naga AM, Aldujaily SS. Effect of Glycyrrhiza Glabra as Motility Stimulant on Sperm Function for Patients with Varicocele and Diabetes Before and After In-Vitro Preparation for ICSI. International Journal of Pharmaceutical Research (09752366). 2020 Oct 1;12(4).
https://openurl.ebsco.com/10.31838/ijpr/2020.12.04.436
Dutta S, Henkel R, Agarwal A. Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation. Andrologia. 2021 Mar;53(2):e13718.
https://doi.org/10.1111/and.13718
Al-Dujaily SS, Selman MO, AL-Hadithy RS, Abdul-Ghafoor HA. Impact of in vitro Preparation with Citrullus colocynthis on DNA Normality of Human Asthenozoospermic Semen. Iraqi J Emb Infertil Res. 2015;5.
Fernandes ND, Da Silva CG, Panizzon GP, Cerialle PM, Câmara VC, Radaelli MR, Almodin CG. Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization. JBRA Assisted Reproduction. 2023 Jan;27(1):25.
https://doi.org/10.5935%2F1518-0557.20220008
Dai C, Zhang Z, Shan G, Chu LT, Huang Z, Moskovtsev S, Librach C, Jarvi K, Sun Y. Advances in sperm analysis: techniques, discoveries and applications. Nature Reviews Urology. 2021 Aug;18(8):447-67.
https://doi.org/10.1038/s41585-021-00472-2
Al-Dujaily SS, Ali SM. DNA normality after in vitro preparation with pentoxifulline and Glycyrrhiza glabra of cryopreserved human sperms.
http://www.wjpr.net/dashboard/abstract_id/2589
Magalon J, Bausset O, Serratrice N, Giraudo L, Aboudou H, Veran J, Magalon G, Dignat-Georges F, Sabatier F. Characterization and comparison of 5 platelet-rich plasma preparations in a single-donor model. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014 May 1;30(5):629-38.
https://doi.org/10.1016/j.arthro.2014.02.020
Abduljabar WA, Mossa HA, Abood MS. Beneficial Role of Platelet–Rich Plasma (PRP) combined with Glass Wool Filtration for Best Recovery of Spermatozoa in Infertile Men. European Journal of Clinical Medicine. 2021 Sep 30;2(5):11-3.
https://doi.org/10.24018/clinicmed.2021.2.5.125
Hamdan D, Rahim AI, Al-Kawaz U. In Vitro Studying the Effect of Adding autologous platelet rich plasma (PRP) to the Human semen on the sperm DNA Integrity. Iraqi J. Embryos Infertility Res. 2021 Nov 9;10:90-100.
http://doi.org/10.28969/IJEIR.v10.i2.r7
Bader R, Ibrahim JN, Moussa M, Mourad A, Azoury J, Azoury J, Alaaeddine N. In vitro effect of autologous platelet‐rich plasma on H2O2‐induced oxidative stress in human spermatozoa. Andrology. 2020 Jan;8(1):191-200.
https://doi.org/10.1111/andr.12648
Ko SN, Son JW, Kim GR, Kim MS, Lee YJ, Kim SJ, Shin JH, Bok WY, Oh HG, Kwon HW. The Inhibitory Effects of Glycyrrhiza uralensis on human Platelet Aggregation and Thrombus Formation. Biomedical Science Letters. 2023 Dec 31;29(4):242-8.
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2025 Noor Kadhim, Saad S. Al-Dujaily , Laith Amer Al-Anbari
This work is licensed under a Creative Commons Attribution 4.0 International License.
