The Influence of DNA Fragmentation Index on Sperm Morphology in Iraqi Men
DOI:
https://doi.org/10.28969/IJEIR.v14.i2.r3.24Keywords:
sperm DFI, sperm morphology, male infertilityAbstract
The assessment of sperm structural abnormalities and sperm DNA damage is very important during the evaluation of male fertility potential. Sperm DNA fragmentation (SDF) is correlated with apoptosis and dysfunction of sperm mitochondrial membrane potential, which is in turn negatively related to motility and normal morphology. The semen sample is collected in a sterile collection container and incubated for 30 minutes at 37°C to allow for liquefaction. Then samples were divided in to three groups P, G and N depending on percentage of morphology. Acidic dyes with a high affinity for non-lysine-rich histones, aniline blue staining (AB), were used to quantify chromatin condensation. AB was used to measure the DNA maturity of spermatozoa. The mean of sperm concentration, total sperm count, total motility, and total progressive motile sperm were more than reference range of WHO 2010. DNA fragmentation index was significantly different (P= 0.003) between the morphology patterns of semen, and it was significantly higher among patients with pattern P than patients with patterns G and N.
In conclusion, as DNA fragmentation is independent test this study showed that low percentage of normal sperm morphology might indicate DNA integrity status.
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