Iraqi Journal of Embryos and Infertility Researches

Protective Influence of Zinc sulphate on some histological changes in Male mice embryo belongs to mother injected with lead acetate

2025-02-24T16:58:15+00:00

Abstract:

Background:

Lead is a heavy metal toxicant, present widely in our environment and workplaces. Zinc has been shown to reduce the toxicity of lead. The present experimental animal study was designed to observe the changes in the testes due to excessive use of lead. This study aims to detect the effect of antioxidant (zinc) on the toxicity of high dose of lead on the reproductive organs of male mice embryo compared to the control group, as a model for human representative.

Materials and methods:

Thirty mature female mice were used (Swiss-Webster), as a mammalian model. They were divided into three groups, 10 mice/group after labeling them. They were randomly assigned into two experimental groups (G1) was injected with 50 mg/kg of body weight of lead acetate from the first day for three days. While (G2) was given 0.5 mg zinc/kg b.w./d orally as zinc sulphate during the 3 weeks period of the experiment(gestation)from the first day of gestation for 3 days, and One control group G3 (n= 10) received distilled water and. Total number of embryos and there weight were recorded for all groups. Then the testes of these embryos excised and processed for histological observations.

Results:

The recorded results showed significant decrease in the weight of the embryo of treated group compared with control group. However, no significant differences in number of the fetus for treated group compared to control one. The histological features of the embryo testes of treated group showed irregular testes cords with prominent degenerative changes and undescended testes compared to that of control group numbers of the embryos belongs to mother Injection with lead acetate and orally administrated with zinc sulphate showed no significant changes in comparison to control group. When mice are administrated to lead for three days, the testicular cords of their testes are disarranged, the primordial germ cells are severe degeneration and descending of the testes remained adjacent to the kidneys (metanephron) at the upper part of the abdominal cavity in comparison with control group.

Conclusion:

It is now generally accepted that the mammalian testes are very sensitive to lead, leading to changes in the testicular biochemical function. Testicular morphology was severely affected in embryo of mice Lead induces production of ROS and reduces the activity of ant oxidative enzymes, thereby causing oxidative damage to the testes. Testicular morphology was severely affected in embryo of mice exposed to lead acetate. Zinc could significantly compete for and effectively reduce the availability of binding sites for lead acetate uptake. Therefore, it plays important role in preventing lead induced reproductive or testes damage in mice. The recorded results of this study assess the presence of teratogenic effect of lead on the developing testes and position of testes of the mice exposed to leads.

The Influence of Density Gradient Technique and Autologous Platelet Rich Plasma Mixed with Glycyrrhiza glabra Medium on DNA Fragmentation Index of Teratozoospermic Men

2025-02-24T16:58:07+00:00

Abstract

Background: Male infertility is a global health issue affecting over 20 million males. A spermatogenesis-related cell differentiation abnormality causes teratozoospermia (TZS). Extensive research has linked TZS to poor fertilization outcomes at assisted reproductive technology (ART) centers as well as to spontaneous pregnancies.

Objective: This study used Glycyrrhiza glabra (Gg) and Platelet Rich Plasma (PRP) media to establish a culture medium that aimed to improve certain sperm functions of teratozoospermic men in vitro.

Methods: A total of fifty semen samples were examined. The World Health Organization 2021 and 1999 standards were used to evaluate certain sperm parameters and the DNA fragmentation index. Platelet Rich Plasma and Gg extract were utilized as a medium for in vitro preparation and activation of semen samples complaining of teratozoospermia factor.

Results: Following activation by the utilization of the density gradient approach and the combination of Gg and PRP medium, there was a statistically a significant (p <0.001) improvement in certain sperm function metrics. These improvements included a higher percentage of morphologically normal sperm, a rise in active motility, and a drop in the DNA fragmentation index.

Conclusion: The density gradient technique, regardless of the medium used in this investigation, resulted in a significant enhancement of morphologically normal sperms with other certain sperm parameters and a decrease in the DNA fragmentation index.

Keywords: Teratozoospermia, DNA fragmentation index, Density gradient technique, Platelet Rich Plasma, Glycyrrhiza glabra

The Influence of DNA Fragmentation Index on Sperm Morphology in Iraqi Men

2025-02-24T16:58:00+00:00

The assessment of sperm structural abnormalities and sperm DNA damage is very important during the evaluation of male fertility potential. Sperm DNA fragmentation (SDF) is correlated with apoptosis and dysfunction of sperm mitochondrial membrane potential, which is in turn negatively related to motility and normal morphology. The semen sample is collected in a sterile collection container and incubated for 30 minutes at 37°C to allow for liquefaction. Then samples were divided in to three groups P, G and N depending on percentage of morphology. Acidic dyes with a high affinity for non-lysine-rich histones, aniline blue staining (AB), were used to quantify chromatin condensation. AB was used to measure the DNA maturity of spermatozoa. The mean of sperm concentration, total sperm count, total motility, and total progressive motile sperm were more than reference range of WHO 2010. DNA fragmentation index was significantly different (P= 0.003) between the morphology patterns of semen, and it was significantly higher among patients with pattern P than patients with patterns G and N.

In conclusion, as DNA fragmentation is independent test this study showed that low percentage of normal sperm morphology might indicate DNA integrity status.

Relationship between Serum Anti-mullerian Hormone and Sperms Characteristics in Infertile Iraqi Men

2025-02-27T17:11:49+00:00

Objective: The objective of the research was to investigate the potential correlation between serum anti-Müllerian hormone (AMH) levels and sperm parameters such as sperm concentration, sperm count, and sperm motility and sperms morphology.

Participants/materials, methods:100 male patients voluntarily participated in the study at a private andrology clinic in Al Najaf Al Ashraf city, Iraq, between October 2023 and November 2023. The patients were divided into four groups: 26 patients with oligozoospermia, 21 patients with asthenozoospermia, 28 patients with oligoasthenoteratozoospermia (OAT), and those with normozoospermia. Semen quality was evaluated by conducting a semen analysis according to with the 2021 World Health Organization guidelines. The ELISA method was employed to measure the levels of AMH in serum samples. Biotechnology Instrument, USA, manufactured the ELIZA components that were used.

Results: The average age of the participants was 30.85 ± 0.70 years, BMI was 27.60 ± 0.58 kg/m² and serum AMH was 2.08 ± 0.11 ng/ml. We did not find any negative or positive correlation between AMH levels in the blood with sperm concentration, sperm count, sperm motility, and sperm morphology in all study groups.

Conclusion: The results of this study showed that serum AMH is an unreliable marker for predicting spermatogenic abnormalities.

Key words: anti-Mullerian hormone / seminal plasma / sperm count / sperm motility / serum / sertoli cells.